› 6x1s

X-ray diffraction
1.65Å resolution

Structure of pHis Fab (SC1-1) in complex with pHis mimetic peptide

Released:
DOI: 10.2210/pdb6x1s/pdb
PDB entry 6x1s coloured by chain, front view.
PDB entry 6x1s coloured by chain, side view.
PDB entry 6x1s coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for differential recognition of phosphohistidine-containing peptides by 1-pHis and 3-pHis monoclonal antibodies.
Kalagiri R, Stanfield RL, Meisenhelder J, La Clair JJ, Fuhs SR, Wilson IA, Hunter T
Proc Natl Acad Sci U S A 118 (2021)
PMID: 33547238

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-211771 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
SC1-1 Heavy chain Chains: A, C, E, H
Molecule details ›
Chains: A, C, E, H
Length: 228 amino acids
Theoretical weight: 23.91 KDa
Source organism: Oryctolagus cuniculus
Expression system: Homo sapiens
SC1-1 Light chain Chains: B, D, F, L
Molecule details ›
Chains: B, D, F, L
Length: 214 amino acids
Theoretical weight: 22.69 KDa
Source organism: Oryctolagus cuniculus
Expression system: Homo sapiens
NM23-1-pTza peptide Chains: G, I, J, K
Molecule details ›
Chains: G, I, J, K
Length: 9 amino acids
Theoretical weight: 1.08 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand MPD 8 x MPD
Ligand SO4 3 x SO4
1 modified residue:
Ligand PCA 4 x PCA

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P2
Unit cell:
a: 86.79Å b: 72.251Å c: 156.747Å
α: 90° β: 95.83° γ: 90°
R-values:
R R work R free
0.201 0.2 0.226
Expression systems:
  • Homo sapiens
  • Not provided

Quick links

Citations

4 review citations

Enzymology and significance of protein histidine methylation.
Jakobsson ME. (2021)
3 more