› 6x2d

X-ray diffraction
1.85Å resolution

Crystal Structure of DNase I Domain of Ribonuclease E from Vibrio cholerae

Released:
DOI: 10.2210/pdb6x2d/pdb
Model geometry
Fit model/data
PDB entry 6x2d coloured by chain, front view.
PDB entry 6x2d coloured by chain, side view.
PDB entry 6x2d coloured by chain, top view.
Source organism: Vibrio cholerae O1 biovar El Tor str. N16961
Entry authors: Minasov G, Shuvalova L, Kiryukhina O, Dubrovska I, Wiersum G, Satchell KJF, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage of single-stranded RNA in A- and U-rich regions
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192030 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonuclease E Chains: A, B
Molecule details ›
Chains: A, B
Length: 115 amino acids
Theoretical weight: 12.97 KDa
Source organism: Vibrio cholerae O1 biovar El Tor str. N16961
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9KQG8 (Residues: 279-390; Coverage: 11%)
Gene names: VC_2030, rne
Sequence domains: Ribonuclease E/G family

Ligands and Environments

1 bound ligand:
Ligand IOD 7 x IOD
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P21
Unit cell:
a: 40.614Å b: 58.812Å c: 47.046Å
α: 90° β: 108.16° γ: 90°
R-values:
R R work R free
0.208 0.207 0.243
Expression system: Escherichia coli BL21(DE3)

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