› 6x4g

X-ray diffraction
3.5Å resolution

Crystal structure of ICOS in complex with ICOS-L and an anti ICOS-L VNAR domain

Released:
DOI: 10.2210/pdb6x4g/pdb
PDB entry 6x4g coloured by chain, front view.
PDB entry 6x4g coloured by chain, side view.
PDB entry 6x4g coloured by chain, top view.
Source organisms:
Primary publication:
Structural characterization of the ICOS/ICOS-L immune complex reveals high molecular mimicry by therapeutic antibodies.
Rujas E, Cui H, Sicard T, Semesi A, Julien JP
Nat Commun 11 5066 (2020)
PMID: 33033255

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-130847 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (4 distinct):
Inducible T-cell costimulator Chain: A
Molecule details ›
Chain: A
Length: 119 amino acids
Theoretical weight: 13.41 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q9Y6W8 (Residues: 19-129; Coverage: 61%)
Gene names: AILIM, ICOS
Sequence domains: ICOS V-set domain
ICOS ligand Chain: C
Molecule details ›
Chain: C
Length: 240 amino acids
Theoretical weight: 26.77 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: O75144 (Residues: 19-248; Coverage: 81%)
Gene names: B7H2, B7RP1, ICOSL, ICOSLG, KIAA0653
Sequence domains: Immunoglobulin V-set domain
anti ICOS-L VHH domain VNAR Chain: N
Molecule details ›
Chain: N
Length: 130 amino acids
Theoretical weight: 13.99 KDa
Source organism: Orectolobus maculatus
Expression system: Homo sapiens

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
2 bound ligands:
Ligand NAG 6 x NAG
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P41212
Unit cell:
a: 104.015Å b: 104.015Å c: 123.108Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.226 0.224 0.266
Expression system: Homo sapiens

Quick links

Citations

6 review citations

Combinatorial Approaches for Cancer Treatment Using Oncolytic Viruses: Projecting the Perspectives through Clinical Trials Outcomes.
Malogolovkin et al. (2021)
5 more