› 6x5r

X-ray diffraction
2.4Å resolution

Human Alpha-1,6-fucosyltransferase (FUT8) bound to GDP and A2-Asn

Released:
DOI: 10.2210/pdb6x5r/pdb
PDB entry 6x5r coloured by chain, front view.
PDB entry 6x5r coloured by chain, side view.
PDB entry 6x5r coloured by chain, top view.
Source organisms:
Primary publication:
Characterizing human α-1,6-fucosyltransferase (FUT8) substrate specificity and structural similarities with related fucosyltransferases.
Boruah BM, Kadirvelraj R, Liu L, Ramiah A, Li C, Zong G, Bosman GP, Yang JY, Wang LX, Boons GJ, Wood ZA, Moremen KW
J Biol Chem 295 17027-17045 (2020)
PMID: 33004438

Function and Biology Details

Reaction catalysed: 
GDP-beta-L-fucose + N(4)-(N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->3)-(N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->6))-beta-D-mannosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-beta-D-glucosaminyl)asparagine = GDP + N(4)-(N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->3)-(N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->6))-beta-D-mannosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-(alpha-L-fucosyl-(1->6))-N-acetyl-beta-D-glucosaminyl)asparagine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-167747 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (3 distinct):
Alpha-(1,6)-fucosyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 535 amino acids
Theoretical weight: 61.85 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q9BYC5 (Residues: 41-575; Coverage: 93%)
Gene name: FUT8
Sequence domains:
A2-Asn Chains: C, D
Molecule details ›
Chains: C, D
Length: 3 amino acids
Theoretical weight: 332 Da
Source organism: Gallus gallus

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
4 bound ligands:
Ligand EDO 4 x EDO
Ligand MN 2 x MN
Ligand GOL 1 x GOL
Ligand GDP 2 x GDP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: P6522
Unit cell:
a: 149.88Å b: 149.88Å c: 480.88Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.207 0.205 0.231
Expression system: Homo sapiens

Quick links

Citations

4 review citations

FUT8 Alpha-(1,6)-Fucosyltransferase in Cancer.
Bastian et al. (2021)
3 more

2 mentions without citation

Structural Insights in Mammalian Sialyltransferases and Fucosyltransferases: We Have Come a Long Way, but It Is Still a Long Way Down.
Grewal et al. (2021)
1 more