› 6x6c

Electron Microscopy
2.9Å resolution

Cryo-EM structure of NLRP1-DPP9-VbP complex

Released:
DOI: 10.2210/pdb6x6c/pdb
PDB entry 6x6c coloured by chain, front view.
PDB entry 6x6c coloured by chain, side view.
PDB entry 6x6c coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
DPP9 sequesters the C terminus of NLRP1 to repress inflammasome activation.
Hollingsworth LR, Sharif H, Griswold AR, Fontana P, Mintseris J, Dagbay KB, Paulo JA, Gygi SP, Bachovchin DA, Wu H
Nature 592 778-783 (2021)
PMID: 33731932
Related structures: EMD-22075

Function and Biology Details

Reaction catalysed: 
Release of an N-terminal dipeptide, Xaa-Yaa-|-Zaa-, from a polypeptide, preferentially when Yaa is Pro, provided Zaa is neither Pro nor hydroxyproline.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183165 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Dipeptidyl peptidase 9 Chains: A, D
Molecule details ›
Chains: A, D
Length: 891 amino acids
Theoretical weight: 101.76 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q86TI2 (Residues: 1-863; Coverage: 100%)
Gene names: DPP9, DPRP2
Sequence domains:
NACHT, LRR and PYD domains-containing protein 1 Chains: E, F
Molecule details ›
Chains: E, F
Length: 1473 amino acids
Theoretical weight: 166.07 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q9C000 (Residues: 1-1473; Coverage: 100%)
Gene names: CARD7, DEFCAP, KIAA0926, NAC, NALP1, NLRP1
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand GK2 2 x GK2
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.9Å
Relevant EMDB volumes: EMD-22075
Expression system: Homo sapiens

Quick links

Citations

34 review citations

Innate Sensors Trigger Regulated Cell Death to Combat Intracellular Infection.
Nozaki et al. (2022)
33 more

3 mentions without citation

DPP9 sequesters the C terminus of NLRP1 to repress inflammasome activation.
Hollingsworth et al. (2021)
2 more