6xav

Electron Microscopy
7.7Å resolution

CryoEM Structure of E. coli Rho-dependent Transcription Pre-termination Complex bound with NusG

Released:
Source organism: Escherichia coli K-12
Related structures: EMD-22115

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero hexadecamer (preferred)
PDBe Complex ID:
PDB-CPX-120926 (preferred)
Entry contents:
7 distinct polypeptide molecules
2 distinct DNA molecules
1 distinct RNA molecule
Macromolecules (10 distinct):
Transcription termination/antitermination protein NusG Chain: L
Molecule details ›
Chain: L
Length: 181 amino acids
Theoretical weight: 20.56 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AFG0 (Residues: 1-181; Coverage: 100%)
Gene names: JW3945, b3982, nusG
Sequence domains:
DNA-directed RNA polymerase subunit omega Chain: W
Molecule details ›
Chain: W
Length: 91 amino acids
Theoretical weight: 10.25 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A800 (Residues: 1-91; Coverage: 100%)
Gene names: JW3624, b3649, rpoZ
Sequence domains: RNA polymerase Rpb6
DNA-directed RNA polymerase subunit alpha Chains: H, K
Molecule details ›
Chains: H, K
Length: 329 amino acids
Theoretical weight: 36.56 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A7Z4 (Residues: 1-329; Coverage: 100%)
Gene names: JW3257, b3295, pez, phs, rpoA, sez
Sequence domains:
DNA-directed RNA polymerase subunit beta Chain: I
Molecule details ›
Chain: I
Length: 1342 amino acids
Theoretical weight: 150.82 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A8V2 (Residues: 1-1342; Coverage: 100%)
Gene names: JW3950, b3987, groN, nitB, rif, ron, rpoB, stl, stv, tabD
Sequence domains:
DNA-directed RNA polymerase subunit beta' Chain: J
Molecule details ›
Chain: J
Length: 1416 amino acids
Theoretical weight: 156.5 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A8T7 (Residues: 2-1407; Coverage: 100%)
Gene names: JW3951, b3988, rpoC, tabB
Sequence domains:
Transcription termination/antitermination protein NusA Chain: G
Molecule details ›
Chain: G
Length: 495 amino acids
Theoretical weight: 54.93 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AFF6 (Residues: 1-495; Coverage: 100%)
Gene names: JW3138, b3169, nusA
Sequence domains:
Transcription termination factor Rho Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 419 amino acids
Theoretical weight: 47.07 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AG30 (Residues: 1-419; Coverage: 100%)
Gene names: JW3756, b3783, nitA, psuA, rho, rnsC, sbaA, tsu
Sequence domains:
DNA (29-MER) Chain: N
Molecule details ›
Chain: N
Length: 31 nucleotides
Theoretical weight: 9.43 KDa
DNA (29-MER) Chain: T
Molecule details ›
Chain: T
Length: 29 nucleotides
Theoretical weight: 8.81 KDa
RNA (18-MER) Chain: R
Molecule details ›
Chain: R
Length: 18 nucleotides
Theoretical weight: 5.86 KDa

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 7.7Å
Relevant EMDB volumes: EMD-22115
Expression system: Escherichia coli