› 6xbd

Electron Microscopy
3.05Å resolution

Cryo-EM structure of MlaFEDB in nanodiscs with phospholipid substrates

Released:
DOI: 10.2210/pdb6xbd/pdb
PDB entry 6xbd coloured by chain, front view.
PDB entry 6xbd coloured by chain, side view.
PDB entry 6xbd coloured by chain, top view.
Source organisms:
Primary publication:
Structure of bacterial phospholipid transporter MlaFEDB with substrate bound.
Coudray N, Isom GL, MacRae MR, Saiduddin MN, Bhabha G, Ekiert DC
Elife 9 (2020)
PMID: 33236984
Related structures: EMD-22116

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetradecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-167767 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Phospholipid ABC transporter-binding protein MlaD Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 201 amino acids
Theoretical weight: 21.94 KDa
Source organism: Escherichia coli DEC6A
Expression system: Escherichia coli
Phospholipid ABC transporter permease protein MlaE Chains: G, H
Molecule details ›
Chains: G, H
Length: 260 amino acids
Theoretical weight: 27.89 KDa
Source organism: Escherichia coli DEC6A
Expression system: Escherichia coli
Phospholipid transport system ATP-binding protein MlaF Chains: I, J
Molecule details ›
Chains: I, J
Length: 269 amino acids
Theoretical weight: 29.13 KDa
Source organism: Escherichia coli DEC6A
Expression system: Escherichia coli
Phospholipid ABC transporter-binding protein MlaB Chains: K, L
Molecule details ›
Chains: K, L
Length: 97 amino acids
Theoretical weight: 10.69 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
MSP1D1 Chains: M, N
Molecule details ›
Chains: M, N
Length: 164 amino acids
Theoretical weight: 13.98 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli

Ligands and Environments

1 bound ligand:
Ligand PEF 2 x PEF
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.05Å
Relevant EMDB volumes: EMD-22116
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Physical properties of the bacterial outer membrane.
Sun et al. (2022)
5 more

10 mentions without citation

Structure, Assembly, and Function of Tripartite Efflux and Type 1 Secretion Systems in Gram-Negative Bacteria.
Alav et al. (2021)
9 more