6xg8

X-ray diffraction
3.5Å resolution

ISCth4 transposase, pre-cleaved complex, PCC

Released:

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-107015 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Mutator family transposase Chains: A, B
Molecule details ›
Chains: A, B
Length: 410 amino acids
Theoretical weight: 47.5 KDa
Source organism: Acetivibrio thermocellus ATCC 27405
Expression system: Escherichia coli
UniProt:
  • Canonical: A3DBR0 (Residues: 1-407; Coverage: 100%)
Gene names: Cthe_0148, Cthe_0356, Cthe_0371, Cthe_1193, Cthe_1874, Cthe_3051
Sequence domains: Transposase, Mutator family
DNA (26-MER) Chain: D
Molecule details ›
Chain: D
Length: 26 nucleotides
Theoretical weight: 7.91 KDa
Source organism: Acetivibrio thermocellus ATCC 27405
Expression system: Not provided
DNA (26-MER) Chain: E
Molecule details ›
Chain: E
Length: 26 nucleotides
Theoretical weight: 8.06 KDa
Source organism: Acetivibrio thermocellus ATCC 27405
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P212121
Unit cell:
a: 89.68Å b: 109.5Å c: 157.7Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.222 0.221 0.267
Expression systems:
  • Escherichia coli
  • Not provided