› 6xl6

Electron Microscopy
3Å resolution

Cryo-EM structure of EcmrR-DNA complex in EcmrR-RPo

Released:
DOI: 10.2210/pdb6xl6/pdb
PDB entry 6xl6 coloured by chain, front view.
PDB entry 6xl6 coloured by chain, side view.
PDB entry 6xl6 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structural visualization of transcription activated by a multidrug-sensing MerR family regulator.
Yang Y, Liu C, Zhou W, Shi W, Chen M, Zhang B, Schatz DG, Hu Y, Liu B
Nat Commun 12 2702 (2021)
PMID: 33976201
Related structures: EMD-22235

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-120961 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
MerR family transcriptional regulator EcmrR Chains: G, H
Molecule details ›
Chains: G, H
Length: 268 amino acids
Theoretical weight: 31.39 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
synthetic non-template strand DNA (54-MER) Chain: N
Molecule details ›
Chain: N
Length: 54 nucleotides
Theoretical weight: 16.72 KDa
Source organism: Escherichia coli
Expression system: Not provided
synthetic template strand DNA (54-MER) Chain: T
Molecule details ›
Chain: T
Length: 54 nucleotides
Theoretical weight: 16.63 KDa
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand 1N7 2 x 1N7
Ligand 118 2 x 118
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3Å
Relevant EMDB volumes: EMD-22235
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

1 review citation

Bacterial MerR family transcription regulators: activationby distortion.
Fang et al. (2022)
14 other articles

2 mentions without citation

Using cryo-EM to uncover mechanisms of bacterial transcriptional regulation.
Wood et al. (2021)
1 more