› 6xls

X-ray diffraction
1.8Å resolution

The 1.80 Angstrom crystal structure of galactose oxidase variant with genetically incorporated F2-Tyr272

Released:
DOI: 10.2210/pdb6xls/pdb
PDB entry 6xls coloured by chain, front view.
PDB entry 6xls coloured by chain, side view.
PDB entry 6xls coloured by chain, top view.
Source organism: Fusarium graminearum
Primary publication:
Formation of Monofluorinated Radical Cofactor in Galactose Oxidase through Copper-Mediated C-F Bond Scission.
Li J, Davis I, Griffith WP, Liu A
J Am Chem Soc 142 18753-18757 (2020)
PMID: 33091303

Function and Biology Details

Reaction catalysed: 
D-galactose + O(2) = D-galacto-hexodialdose + H(2)O(2)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143550 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Galactose oxidase Chain: A
Molecule details ›
Chain: A
Length: 648 amino acids
Theoretical weight: 69.84 KDa
Source organism: Fusarium graminearum
Expression system: Escherichia coli
UniProt:
  • Canonical: P0CS93 (Residues: 42-680; Coverage: 97%)
Gene name: GAOA
Sequence domains:

Ligands and Environments

4 bound ligands:
Ligand CU 1 x CU
Ligand CA 1 x CA
Ligand ACT 4 x ACT
Ligand GOL 1 x GOL
1 modified residue:
Ligand F2Y 1 x F2Y

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: C2
Unit cell:
a: 97.228Å b: 89.162Å c: 86.159Å
α: 90° β: 117.94° γ: 90°
R-values:
R R work R free
0.149 0.148 0.184
Expression system: Escherichia coli

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Citations

2 review citations

Nothing lasts forever: understanding microbial biodegradation of polyfluorinated compounds and perfluorinated alkyl substances.
Wackett LP. (2022)
1 more

1 mention without citation

Formation of Monofluorinated Radical Cofactor in Galactose Oxidase through Copper-Mediated C-F Bond Scission.
Li et al. (2020)