› 6xlv

X-ray diffraction
1.4Å resolution

Crystal structure of leukemia-associated N196K mutant of U2AF65 bound to AdML splice site

Released:
DOI: 10.2210/pdb6xlv/pdb
PDB entry 6xlv coloured by chain, front view.
PDB entry 6xlv coloured by chain, side view.
PDB entry 6xlv coloured by chain, top view.
Source organisms:
Primary publication:
Representative cancer-associated U2AF2 mutations alter RNA interactions and splicing.
Maji D, Glasser E, Henderson S, Galardi J, Pulvino MJ, Jenkins JL, Kielkopf CL
J Biol Chem 295 17148-17157 (2020)
PMID: 33020180

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150722 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Splicing factor U2AF 65 kDa subunit Chain: A
Molecule details ›
Chain: A
Length: 204 amino acids
Theoretical weight: 22.25 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P26368 (Residues: 141-341; Coverage: 42%)
Gene names: U2AF2, U2AF65
Sequence domains: RNA recognition motif
DNA/RNA (5'-R(P*UP*UP*(UD)P*UP*U)-D(P*(BRU))-R(P*CP*C)-3') Chain: B
Molecule details ›
Chain: B
Length: 8 nucleotides
Theoretical weight: 2.45 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:
Ligand BRU 1 x BRU

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P212121
Unit cell:
a: 43.335Å b: 63.048Å c: 76.892Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.143 0.141 0.169
Expression systems:
  • Escherichia coli BL21
  • Not provided

Quick links

Citations

6 review citations

CELF Family Proteins in Cancer: Highlights on the RNA-Binding Protein/Noncoding RNA Regulatory Axis.
Nasiri-Aghdam et al. (2021)
5 more

1 mention without citation

Representative cancer-associated U2AF2 mutations alter RNA interactions and splicing.
Maji et al. (2020)