› 6xmf

Electron Microscopy
3.1Å resolution

Cryo-EM structure of Cas12g binary complex

Released:
DOI: 10.2210/pdb6xmf/pdb
PDB entry 6xmf coloured by chain, front view.
PDB entry 6xmf coloured by chain, side view.
PDB entry 6xmf coloured by chain, top view.
Source organism: metagenome
Primary publication:
Cryo-EM structure of the RNA-guided ribonuclease Cas12g.
Li Z, Zhang H, Xiao R, Han R, Chang L
Nat Chem Biol 17 387-393 (2021)
PMID: 33495647
Related structures: EMD-22257

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-215437 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
CRISPR-Cas Chain: A
Molecule details ›
Chain: A
Length: 767 amino acids
Theoretical weight: 89.89 KDa
Source organism: metagenome
Expression system: Escherichia coli BL21(DE3)
RNA (116-MER) Chain: C
Molecule details ›
Chain: C
Length: 122 nucleotides
Theoretical weight: 39.36 KDa
Source organism: metagenome
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.1Å
Relevant EMDB volumes: EMD-22257
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

6 review citations

Structural biology of CRISPR-Cas immunity and genome editing enzymes.
Wang et al. (2022)
5 more

2 mentions without citation

Cryo-EM structure of the RNA-guided ribonuclease Cas12g.
Li et al. (2021)
1 more