› 6xmg

Electron Microscopy
4.8Å resolution

Cryo-EM structure of Cas12g ternary complex

Released:
DOI: 10.2210/pdb6xmg/pdb
PDB entry 6xmg coloured by chain, front view.
PDB entry 6xmg coloured by chain, side view.
PDB entry 6xmg coloured by chain, top view.
Source organism: metagenome
Primary publication:
Cryo-EM structure of the RNA-guided ribonuclease Cas12g.
Li Z, Zhang H, Xiao R, Han R, Chang L
Nat Chem Biol 17 387-393 (2021)
PMID: 33495647
Related structures: EMD-22258

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-215438 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct RNA molecules
Macromolecules (3 distinct):
CRISPR-Cas Chain: A
Molecule details ›
Chain: A
Length: 767 amino acids
Theoretical weight: 89.89 KDa
Source organism: metagenome
Expression system: Escherichia coli BL21(DE3)
RNA (130-MER) Chain: C
Molecule details ›
Chain: C
Length: 136 nucleotides
Theoretical weight: 43.78 KDa
Source organism: metagenome
Expression system: Not provided
RNA (5'-R(P*UP*UP*AP*AP*UP*GP*CP*GP*GP*UP*AP*GP*UP*UP*UP*AP*UP*CP*AP*CP*AP*GP*UP*U)-3') Chain: E
Molecule details ›
Chain: E
Length: 24 nucleotides
Theoretical weight: 7.63 KDa
Source organism: metagenome
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.8Å
Relevant EMDB volumes: EMD-22258
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

6 review citations

Structural biology of CRISPR-Cas immunity and genome editing enzymes.
Wang et al. (2022)
5 more

2 mentions without citation

Cryo-EM structure of the RNA-guided ribonuclease Cas12g.
Li et al. (2021)
1 more