› 6xql

X-ray diffraction
1.97Å resolution

Crystal structure of SCLam E144S mutant, a non-specific endo-beta-1,3(4)-glucanase from family GH16, co-crystallized with cellohexaose, presenting a 1,3-beta-D-cellobiosyl-glucose at active site

Released:
DOI: 10.2210/pdb6xql/pdb
Model geometry
Fit model/data
PDB entry 6xql coloured by chain, front view.
PDB entry 6xql coloured by chain, side view.
PDB entry 6xql coloured by chain, top view.
Source organism: uncultured bacterium
Primary publication:
Insights into the dual cleavage activity of the GH16 laminarinase enzyme class on β-1,3 and β-1,4 glycosidic bonds.
Liberato MV, Teixeira Prates E, Gonçalves TA, Bernardes A, Vilela N, Fattori J, Ematsu GC, Chinaglia M, Machi Gomes ER, Migliorini Figueira AC, Damasio A, Polikarpov I, Skaf MS, Squina FM
OpenAccess logo J Biol Chem 296 100385
PMID: 33556371

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-101006 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
GH16 domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 269 amino acids
Theoretical weight: 30.29 KDa
Source organism: uncultured bacterium
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0B5H9B3 (Residues: 1-265; Coverage: 100%)
Gene name: sclam
Sequence domains: Glycosyl hydrolases family 16

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC, BGC
Carbohydrate polymer
1 bound ligand:
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: LNLS BEAMLINE W01B-MX2
Spacegroup: P212121
Unit cell:
a: 38.4Å b: 49.006Å c: 113.995Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.173 0.17 0.239
Expression system: Escherichia coli

Quick links

Citations

3 citation in other articles

New globally distributed bacterial phyla within the FCB superphylum.
Gong et al. (2022)
2 more

1 mention without citation

Insights into the dual cleavage activity of the GH16 laminarinase enzyme class on β-1,3 and β-1,4 glycosidic bonds.
Liberato et al. (2021)