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› 6xs3

X-ray diffraction
2.48Å resolution

X-ray structure of the monoclinic crystal form at 2.48 A resolution of lipase from Thermomyces (Humicola) lanuginosa at 298 K

Released:
DOI: 10.2210/pdb6xs3/pdb
Model geometry
Fit model/data
PDB entry 6xs3 coloured by chain, front view.
PDB entry 6xs3 coloured by chain, side view.
PDB entry 6xs3 coloured by chain, top view.
Source organism: Thermomyces lanuginosus
Primary publication:
The crystal Structures of Thermomyces (Humicola) lanuginosa lipase in complex with enzymatic reactants
McPherson A, Larson SB, Kalasky A
Current Enzyme Inhibition 16 (2020)

Function and Biology Details

Reaction catalysed: 
a triacylglycerol + H2O = a diacylglycerol + a fatty acid + H(+).
Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129892 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lipase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 291 amino acids
Theoretical weight: 31.84 KDa
Source organism: Thermomyces lanuginosus
Expression system: Aspergillus aculeatinus
UniProt:
  • Canonical: O59952 (Residues: 1-291; Coverage: 100%)
Gene name: LIP
Sequence domains:

Ligands and Environments

5 bound ligands:
Ligand OCA 6 x OCA
Ligand CA 8 x CA
Ligand NAG 6 x NAG
Ligand LTV 6 x LTV
Ligand PO4 5 x PO4
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P21
Unit cell:
a: 78.185Å b: 91.366Å c: 124.316Å
α: 90° β: 94.7° γ: 90°
R-values:
R R work R free
0.18 0.178 0.213
Expression system: Aspergillus aculeatinus

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