› 6xwg

X-ray diffraction
2.4Å resolution

Crystal Structure of the Human RXR/RAR DNA-Binding Domain Heterodimer Bound to the Human RARb2 DR5 Response Element

Released:
DOI: 10.2210/pdb6xwg/pdb
PDB entry 6xwg coloured by chain, front view.
PDB entry 6xwg coloured by chain, side view.
PDB entry 6xwg coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for DNA recognition and allosteric control of the retinoic acid receptors RAR-RXR.
Osz J, McEwen AG, Bourguet M, Przybilla F, Peluso-Iltis C, Poussin-Courmontagne P, Mély Y, Cianférani S, Jeffries CM, Svergun DI, Rochel N
Nucleic Acids Res 48 9969-9985 (2020)
PMID: 32974652

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-120982 (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
Retinoic acid receptor RXR-alpha Chain: C
Molecule details ›
Chain: C
Length: 87 amino acids
Theoretical weight: 10.26 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P19793 (Residues: 130-212; Coverage: 18%)
Gene names: NR2B1, RXRA
Sequence domains: Zinc finger, C4 type (two domains)
Retinoic acid receptor alpha Chain: D
Molecule details ›
Chain: D
Length: 90 amino acids
Theoretical weight: 10.63 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P10276 (Residues: 82-167; Coverage: 19%)
Gene names: NR1B1, RARA
Sequence domains: Zinc finger, C4 type (two domains)
RARb2 DR5 Response Element, 5'-3' strand Chain: A
Molecule details ›
Chain: A
Length: 21 nucleotides
Theoretical weight: 6.45 KDa
RARb2 DR5 Response Element, 3'-5' strand Chain: B
Molecule details ›
Chain: B
Length: 21 nucleotides
Theoretical weight: 6.44 KDa

Ligands and Environments

3 bound ligands:
Ligand ZN 4 x ZN
Ligand GOL 1 x GOL
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P43212
Unit cell:
a: 56.714Å b: 56.714Å c: 238.364Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.191 0.207
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

Retinoid X Receptor: Cellular and Biochemical Roles of Nuclear Receptor with a Focus on Neuropathological Involvement.
Sharma et al. (2022)
6 more

3 mentions without citation

Structural basis for DNA recognition and allosteric control of the retinoic acid receptors RAR-RXR.
Osz et al. (2020)
2 more