› 6xwh

X-ray diffraction
2.1Å resolution

Crystal Structure of the Human RXR DNA-Binding Domain Homodimer Bound to the Human Hoxb13 DR0 Response Element

Released:
DOI: 10.2210/pdb6xwh/pdb
PDB entry 6xwh coloured by chain, front view.
PDB entry 6xwh coloured by chain, side view.
PDB entry 6xwh coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for DNA recognition and allosteric control of the retinoic acid receptors RAR-RXR.
Osz J, McEwen AG, Bourguet M, Przybilla F, Peluso-Iltis C, Poussin-Courmontagne P, Mély Y, Cianférani S, Jeffries CM, Svergun DI, Rochel N
Nucleic Acids Res 48 9969-9985 (2020)
PMID: 32974652

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-120983 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Retinoic acid receptor RXR-alpha Chains: C, D
Molecule details ›
Chains: C, D
Length: 87 amino acids
Theoretical weight: 10.26 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P19793 (Residues: 130-212; Coverage: 18%)
Gene names: NR2B1, RXRA
Sequence domains: Zinc finger, C4 type (two domains)
Hoxb13 DR0 Response Element, 5'-3' strand Chain: A
Molecule details ›
Chain: A
Length: 16 nucleotides
Theoretical weight: 4.98 KDa
Source organism: Homo sapiens
Expression system: Not provided
Hoxb13 DR0 Response Element, 3'-5' strand Chain: B
Molecule details ›
Chain: B
Length: 16 nucleotides
Theoretical weight: 4.82 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ZN 4 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P21
Unit cell:
a: 45.705Å b: 62.522Å c: 53.635Å
α: 90° β: 104.84° γ: 90°
R-values:
R R work R free
0.187 0.185 0.224
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

7 review citations

Retinoid X Receptor: Cellular and Biochemical Roles of Nuclear Receptor with a Focus on Neuropathological Involvement.
Sharma et al. (2022)
6 more

3 mentions without citation

Structural basis for DNA recognition and allosteric control of the retinoic acid receptors RAR-RXR.
Osz et al. (2020)
2 more