› 6xwm

X-ray diffraction
2.6Å resolution

Mechanism of substrate release in neurotransmitter:sodium symporters: the structure of LeuT in an inward-facing occluded conformation

Released:
DOI: 10.2210/pdb6xwm/pdb
PDB entry 6xwm coloured by chain, front view.
PDB entry 6xwm coloured by chain, side view.
PDB entry 6xwm coloured by chain, top view.
Source organism: Aquifex aeolicus VF5
Primary publication:
X-ray structure of LeuT in an inward-facing occluded conformation reveals mechanism of substrate release.
Gotfryd K, Boesen T, Mortensen JS, Khelashvili G, Quick M, Terry DS, Missel JW, LeVine MV, Gourdon P, Blanchard SC, Javitch JA, Weinstein H, Loland CJ, Nissen P, Gether U
Nat Commun 11 1005 (2020)
PMID: 32081981

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130578 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Na(+):neurotransmitter symporter (Snf family) Chains: A, B
Molecule details ›
Chains: A, B
Length: 519 amino acids
Theoretical weight: 57.96 KDa
Source organism: Aquifex aeolicus VF5
Expression system: Escherichia coli
UniProt:
  • Canonical: O67854 (Residues: 1-513; Coverage: 100%)
Gene names: aq_2077, snf
Sequence domains: Sodium:neurotransmitter symporter family

Ligands and Environments

2 bound ligands:
Ligand PHE 2 x PHE
Ligand NA 4 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P1
Unit cell:
a: 77.848Å b: 83.985Å c: 86.939Å
α: 65.38° β: 89.77° γ: 64.56°
R-values:
R R work R free
0.212 0.211 0.233
Expression system: Escherichia coli

Quick links

Citations

9 review citations

SLC6 transporter oligomerization.
Jayaraman et al. (2021)
8 more

2 mentions without citation

Structural basis of GABA reuptake inhibition.
Motiwala et al. (2022)
1 more