› 6xyz

X-ray diffraction
1.63Å resolution

Crystal structure of the GH18 chitinase ChiB from the chitin utilization locus of Flavobacterium johnsoniae

Released:
DOI: 10.2210/pdb6xyz/pdb
Model geometry
Fit model/data
PDB entry 6xyz coloured by chain, front view.
PDB entry 6xyz coloured by chain, side view.
PDB entry 6xyz coloured by chain, top view.
Source organism: Flavobacterium johnsoniae UW101
Primary publication:
Structural insights of the enzymes from the chitin utilization locus of Flavobacterium johnsoniae.
Mazurkewich S, Helland R, Mackenzie A, Eijsink VGH, Pope PB, Brändén G, Larsbrink J
OpenAccess logo Sci Rep 10 13775 (2020)
PMID: 32792608

Function and Biology Details

Reaction catalysed: 
Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-107581 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
chitinase Chain: A
Molecule details ›
Chain: A
Length: 325 amino acids
Theoretical weight: 36.55 KDa
Source organism: Flavobacterium johnsoniae UW101
Expression system: Escherichia coli
UniProt:
  • Canonical: A5FB54 (Residues: 24-340; Coverage: 100%)
Gene name: Fjoh_4560
Sequence domains: Glycosyl hydrolases family 18

Ligands and Environments

2 bound ligands:
Ligand EDO 3 x EDO
Ligand FMT 1 x FMT
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ESRF BEAMLINE MASSIF-3
Spacegroup: P21
Unit cell:
a: 43.91Å b: 66.44Å c: 53.44Å
α: 90° β: 98.307° γ: 90°
R-values:
R R work R free
0.159 0.157 0.189
Expression system: Escherichia coli

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Citations

3 citation in other articles

Mechanism of Cooperative Degradation of Gum Arabic Arabinogalactan Protein by Bifidobacterium longum Surface Enzymes.
Sasaki et al. (2022)
2 more

2 mentions without citation

A chemical interpretation of protein electron density maps in the worldwide protein data bank.
Yao et al. (2020)
1 more