› 6y39

X-ray diffraction
2.3Å resolution

HapE-P88L mutant CCAAT-binding complex from Aspergillus nidulans with cycA DNA

Released:
DOI: 10.2210/pdb6y39/pdb
PDB entry 6y39 coloured by chain, front view.
PDB entry 6y39 coloured by chain, side view.
PDB entry 6y39 coloured by chain, top view.
Source organism: Aspergillus nidulans FGSC A4
Primary publication:
Structural basis of HapEP88L-linked antifungal triazole resistance in Aspergillus fumigatus.
Hortschansky P, Misslinger M, Mörl J, Gsaller F, Bromley MJ, Brakhage AA, Groll M, Haas H, Huber EM
Life Sci Alliance 3 (2020)
PMID: 32467317

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero undecamer (preferred)
PDBe Complex ID:
PDB-CPX-241157 (preferred)
Entry contents:
3 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (5 distinct):
Transcriptional activator HAP2 Chains: A, D, G
Molecule details ›
Chains: A, D, G
Length: 64 amino acids
Theoretical weight: 7.67 KDa
Source organism: Aspergillus nidulans FGSC A4
Expression system: Escherichia coli
UniProt:
  • Canonical: G5EAZ0 (Residues: 231-293; Coverage: 17%)
Gene name: ANIA_07545
Sequence domains: CCAAT-binding transcription factor (CBF-B/NF-YA) subunit B
Transcription factor CBF/NF-Y/archaeal histone domain-containing protein Chains: B, E, H
Molecule details ›
Chains: B, E, H
Length: 92 amino acids
Theoretical weight: 10.64 KDa
Source organism: Aspergillus nidulans FGSC A4
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5B5Z6 (Residues: 41-132; Coverage: 42%)
Gene name: ANIA_04034
Sequence domains: Histone-like transcription factor (CBF/NF-Y) and archaeal histone
CBFD_NFYB_HMF domain-containing protein Chains: C, F, I
Molecule details ›
Chains: C, F, I
Length: 119 amino acids
Theoretical weight: 13.68 KDa
Source organism: Aspergillus nidulans FGSC A4
Expression system: Escherichia coli
DNA (25 mer) Chain: J
Molecule details ›
Chain: J
Length: 25 nucleotides
Theoretical weight: 7.57 KDa
DNA (25 mer) Chain: K
Molecule details ›
Chain: K
Length: 25 nucleotides
Theoretical weight: 7.78 KDa

Ligands and Environments

1 bound ligand:
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P212121
Unit cell:
a: 72.15Å b: 103.5Å c: 159.71Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.222 0.221 0.254
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Aspergillosis, Avian Species and the One Health Perspective: The Possible Importance of Birds in Azole Resistance.
Melo et al. (2020)
2 more

1 mention without citation

Structural basis of HapEP88L-linked antifungal triazole resistance in Aspergillus fumigatus.
Hortschansky et al. (2020)