6y3r

X-ray diffraction
1.5Å resolution

14-3-3 Sigma in complex with phosphorylated (Thr391) Gab2 peptide

Released:
Source organism: Homo sapiens
Primary publication:
A new soaking procedure for X-ray crystallographic structural determination of protein-peptide complexes.
Acta Crystallogr F Struct Biol Commun 76 501-507 (2020)
PMID: 33006579

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-152172 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
14-3-3 protein sigma Chain: A
Molecule details ›
Chain: A
Length: 253 amino acids
Theoretical weight: 28.23 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P31947 (Residues: 1-248; Coverage: 100%)
Gene names: HME1, SFN
Sequence domains: 14-3-3 protein
GRB2-associated-binding protein 2 Chain: P
Molecule details ›
Chain: P
Length: 12 amino acids
Theoretical weight: 1.45 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q9UQC2 (Residues: 387-398; Coverage: 2%)
Gene names: GAB2, KIAA0571

Ligands and Environments

1 bound ligand:
2 modified residues:

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: C2221
Unit cell:
a: 82.438Å b: 111.896Å c: 62.349Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 0.182 0.22
Expression systems:
  • Escherichia coli
  • Not provided