› 6y42

X-ray diffraction
4.3Å resolution

Crystal Structure of RsrR complexed to a 39 basepair DNA fragment of the rsrR promoter

Released:
DOI: 10.2210/pdb6y42/pdb
PDB entry 6y42 coloured by chain, front view.
PDB entry 6y42 coloured by chain, side view.
PDB entry 6y42 coloured by chain, top view.
Source organism: Streptomyces venezuelae ATCC 10712
Primary publication:
Electron and Proton Transfers Modulate DNA Binding by the Transcription Regulator RsrR.
Crack JC, Amara P, Volbeda A, Mouesca JM, Rohac R, Pellicer Martinez MT, Huang CY, Gigarel O, Rinaldi C, Le Brun NE, Fontecilla-Camps JC
J Am Chem Soc 142 5104-5116 (2020)
PMID: 32078310

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-120986 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Rrf2 family transcriptional regulator Chains: A, B
Molecule details ›
Chains: A, B
Length: 166 amino acids
Theoretical weight: 17.39 KDa
Source organism: Streptomyces venezuelae ATCC 10712
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: F2RGC9 (Residues: 1-160; Coverage: 100%)
Gene name: SVEN_6563
Sequence domains: Iron-dependent Transcriptional regulator
DNA (39-MER) Chain: E
Molecule details ›
Chain: E
Length: 39 nucleotides
Theoretical weight: 12.13 KDa
Source organism: Streptomyces venezuelae ATCC 10712
Expression system: Not provided
DNA (39-MER) Chain: F
Molecule details ›
Chain: F
Length: 39 nucleotides
Theoretical weight: 11.88 KDa
Source organism: Streptomyces venezuelae ATCC 10712
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand FES 2 x FES
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P6122
Unit cell:
a: 197.37Å b: 197.37Å c: 73.19Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.268 0.267 0.3
Expression systems:
  • Escherichia coli 'BL21-Gold(DE3)pLysS AG'
  • Not provided

Quick links

Citations

5 citation in other articles

Structural basis for persulfide-sensing specificity in a transcriptional regulator.
Capdevila et al. (2021)
4 more

1 mention without citation

Structural determinants of DNA recognition by the NO sensor NsrR and related Rrf2-type [FeS]-transcription factors.
Rohac et al. (2022)