› 6y4p

X-ray diffraction
2.13Å resolution

Calmodulin N53I variant bound to cardiac ryanodine receptor (RyR2) calmodulin binding domain

Released:
DOI: 10.2210/pdb6y4p/pdb
PDB entry 6y4p coloured by chain, front view.
PDB entry 6y4p coloured by chain, side view.
PDB entry 6y4p coloured by chain, top view.
Source organisms:
Primary publication:
The arrhythmogenic N53I variant subtly changes the structure and dynamics in the calmodulin N-terminal domain, altering its interaction with the cardiac ryanodine receptor.
Holt C, Hamborg L, Lau K, Brohus M, Sørensen AB, Larsen KT, Sommer C, Van Petegem F, Overgaard MT, Wimmer R
J Biol Chem 295 7620-7634 (2020)
PMID: 32317284

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143955 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Calmodulin-1 Chain: A
Molecule details ›
Chain: A
Length: 149 amino acids
Theoretical weight: 16.85 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P0DP23 (Residues: 1-149; Coverage: 100%)
Gene names: CALM, CALM1, CAM, CAM1
Sequence domains: EF-hand domain pair
Ryanodine receptor 2 Chain: B
Molecule details ›
Chain: B
Length: 30 amino acids
Theoretical weight: 3.48 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q92736 (Residues: 3581-3607; Coverage: 1%)
Gene name: RYR2

Ligands and Environments

1 bound ligand:
Ligand CA 4 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08B1-1
Spacegroup: P212121
Unit cell:
a: 37.59Å b: 42.36Å c: 90.36Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.182 0.246
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Structural Insight Into Ryanodine Receptor Channelopathies.
Hadiatullah et al. (2022)
1 more

1 mention without citation

The arrhythmogenic N53I variant subtly changes the structure and dynamics in the calmodulin N-terminal domain, altering its interaction with the cardiac ryanodine receptor.
Holt et al. (2020)