› 6y91

X-ray diffraction
2.5Å resolution

Crystal structure of malate dehydrogenase from Plasmodium Falciparum in complex with NADH

Released:
DOI: 10.2210/pdb6y91/pdb
Model geometry
Fit model/data
Data not analysed
PDB entry 6y91 coloured by chain, front view.
PDB entry 6y91 coloured by chain, side view.
PDB entry 6y91 coloured by chain, top view.
Source organism: Plasmodium falciparum
Primary publication:
A fragment-based approach identifies an allosteric pocket that impacts malate dehydrogenase activity.
Reyes Romero A, Lunev S, Popowicz GM, Calderone V, Gentili M, Sattler M, Plewka J, Taube M, Kozak M, Holak TA, Dömling ASS, Groves MR
OpenAccess logo Commun Biol 4 949 (2021)
PMID: 34376783

Function and Biology Details

Reaction catalysed: 
(S)-lactate + NAD(+) = pyruvate + NADH
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180475 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-lactate dehydrogenase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 324 amino acids
Theoretical weight: 35.36 KDa
Source organism: Plasmodium falciparum
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q6VVP7 (Residues: 1-313; Coverage: 100%)
Sequence domains:

Ligands and Environments


Cofactor: Ligand NAD 4 x NAD
No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: BRUKER D8 QUEST
Spacegroup: P212121
Unit cell:
a: 84.62Å b: 107.42Å c: 145.08Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.241 0.239 0.286
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Malaria therapeutics: are we close enough?
Tripathi et al. (2023)

1 mention without citation

A fragment-based approach identifies an allosteric pocket that impacts malate dehydrogenase activity.
Reyes Romero et al. (2021)