› 6y93

X-ray diffraction
2.23Å resolution

Crystal structure of the DNA-binding domain of the Nucleoid Occlusion Factor (Noc) complexed to the Noc-binding site (NBS)

Released:
DOI: 10.2210/pdb6y93/pdb
PDB entry 6y93 coloured by chain, front view.
PDB entry 6y93 coloured by chain, side view.
PDB entry 6y93 coloured by chain, top view.
Source organisms:
Primary publication:
Diversification of DNA-Binding Specificity by Permissive and Specificity-Switching Mutations in the ParB/Noc Protein Family.
Jalal ASB, Tran NT, Stevenson CE, Chan EW, Lo R, Tan X, Noy A, Lawson DM, Le TBK
Cell Rep 32 107928 (2020)
PMID: 32698006

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-120989 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Nucleoid occlusion protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 146 amino acids
Theoretical weight: 16.81 KDa
Source organism: Bacillus subtilis subsp. subtilis str. 168
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P37524 (Residues: 111-242; Coverage: 47%)
Gene names: BSU40990, noc, yyaA
Sequence domains: HTH domain found in ParB protein
Noc Binding Site (NBS) Chains: C, D
Molecule details ›
Chains: C, D
Length: 22 nucleotides
Theoretical weight: 6.75 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: C2
Unit cell:
a: 134.139Å b: 60.567Å c: 81.053Å
α: 90° β: 116.88° γ: 90°
R-values:
R R work R free
0.234 0.231 0.279
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

2 review citations

Nucleoid-associated proteins shape chromatin structure and transcriptional regulation across the bacterial kingdom.
Amemiya et al. (2021)
1 more

2 mentions without citation

Diversification of DNA-Binding Specificity by Permissive and Specificity-Switching Mutations in the ParB/Noc Protein Family.
Jalal et al. (2020)
1 more