› 6y98

X-ray diffraction
2.8Å resolution

Crystal Structure of subtype-switched Epithelial Adhesin 9 to 1 A domain (Epa9-CBL2Epa1) from Candida glabrata in complex with beta-lactose

Released:
DOI: 10.2210/pdb6y98/pdb
PDB entry 6y98 coloured by chain, front view.
PDB entry 6y98 coloured by chain, side view.
PDB entry 6y98 coloured by chain, top view.
Source organism: [Candida] glabrata CBS 138
Primary publication:
Functional reprogramming of Candida glabrata epithelial adhesins: the role of conserved and variable structural motifs in ligand binding.
Hoffmann D, Diderrich R, Reithofer V, Friederichs S, Kock M, Essen LO, Mösch HU
J Biol Chem 295 12512-12524 (2020)
PMID: 32669365

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110130 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
PA14 domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 290 amino acids
Theoretical weight: 33.23 KDa
Source organism: [Candida] glabrata CBS 138
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: B4UMX2 (Residues: 39-305; Coverage: 19%)
Gene names: CAGL0A01366g, EPA9
Sequence domains: GLEYA domain

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC, GAL
Carbohydrate polymer
1 bound ligand:
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P3121
Unit cell:
a: 65.289Å b: 65.289Å c: 121.877Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.206 0.204 0.248
Expression system: Escherichia coli K-12

Quick links

Citations

5 citation in other articles

A novel class of Candida glabrata cell wall proteins with β-helix fold mediates adhesion in clinical isolates.
Reithofer et al. (2021)
4 more

1 mention without citation

Functional reprogramming of Candida glabrata epithelial adhesins: the role of conserved and variable structural motifs in ligand binding.
Hoffmann et al. (2020)