› 6y9b

Electron Microscopy
2.97Å resolution

Cryo-EM structure of trimeric human STEAP1 bound to three Fab120.545 fragments

Released:
DOI: 10.2210/pdb6y9b/pdb
PDB entry 6y9b coloured by chain, front view.
PDB entry 6y9b coloured by chain, side view.
PDB entry 6y9b coloured by chain, top view.
Source organisms:
Primary publication:
Cryo-electron microscopy structure and potential enzymatic function of human six-transmembrane epithelial antigen of the prostate 1 (STEAP1).
Oosterheert W, Gros P
J Biol Chem 295 9502-9512 (2020)
PMID: 32409586
Related structures: EMD-10735

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero nonamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-212832 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
STEAP1 protein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 380 amino acids
Theoretical weight: 44.18 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q9UHE8 (Residues: 2-339; Coverage: 100%)
Gene names: PRSS24, STEAP, STEAP1
Sequence domains: Ferric reductase like transmembrane component
Fab120.545 light chain Chains: L, M, N
Molecule details ›
Chains: L, M, N
Length: 220 amino acids
Theoretical weight: 24.34 KDa
Source organism: Mus musculus
Expression system: Homo sapiens
Fab120.545 heavy chain Chains: H, I, J
Molecule details ›
Chains: H, I, J
Length: 238 amino acids
Theoretical weight: 26.15 KDa
Source organism: Mus musculus
Expression system: Homo sapiens

Ligands and Environments

2 bound ligands:
Ligand HEM 3 x HEM
Ligand XP4 3 x XP4
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.97Å
Relevant EMDB volumes: EMD-10735
Expression system: Homo sapiens

Quick links

Citations

5 review citations

Treating Prostate Cancer by Antibody-Drug Conjugates.
Rosellini et al. (2021)
4 more

3 mentions without citation

Cryo-electron microscopy structure and potential enzymatic function of human six-transmembrane epithelial antigen of the prostate 1 (STEAP1).
Oosterheert et al. (2020)
2 more