› 6y9f

X-ray diffraction
1.26Å resolution

Crystal structure of putative ancestral haloalkane dehalogenase AncHLD3 (node 3)

Released:
DOI: 10.2210/pdb6y9f/pdb
PDB entry 6y9f coloured by chain, front view.
PDB entry 6y9f coloured by chain, side view.
PDB entry 6y9f coloured by chain, top view.
Source organism: synthetic construct
Primary publication:
Structures of hyperstable ancestral haloalkane dehalogenases show restricted conformational dynamics.
Babkova P, Dunajova Z, Chaloupkova R, Damborsky J, Bednar D, Marek M
Comput Struct Biotechnol J 18 1497-1508 (2020)
PMID: 32637047

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-167840 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ancestral haloalkane dehalogenase AncHLD3 Chain: A
Molecule details ›
Chain: A
Length: 307 amino acids
Theoretical weight: 34.07 KDa
Source organism: synthetic construct
Expression system: Escherichia coli

Ligands and Environments

1 bound ligand:
Ligand NHE 2 x NHE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P6522
Unit cell:
a: 71.42Å b: 71.42Å c: 225.76Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.148 0.147 0.169
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Mini Review: Advances in 2-Haloacid Dehalogenases.
Wang et al. (2021)
3 other articles

1 mention without citation

Structures of hyperstable ancestral haloalkane dehalogenases show restricted conformational dynamics.
Babkova et al. (2020)