› 6ybr

X-ray diffraction
1.2Å resolution

RT structure of Glucose Isomerase obtained at 1.20 A resolution from crystal grown in a Mylar microchip.

Released:
DOI: 10.2210/pdb6ybr/pdb
PDB entry 6ybr coloured by chain, front view.
PDB entry 6ybr coloured by chain, side view.
PDB entry 6ybr coloured by chain, top view.
Source organism: Streptomyces rubiginosus
Primary publication:
Attaining atomic resolution from in situ data collection at room temperature using counter-diffusion-based low-cost microchips.
Gavira JA, Rodriguez-Ruiz I, Martinez-Rodriguez S, Basu S, Teychené S, McCarthy AA, Mueller-Dieckman C
Acta Crystallogr D Struct Biol 76 751-758 (2020)
PMID: 32744257

Function and Biology Details

Reaction catalysed: 
Alpha-D-xylopyranose = alpha-D-xylufuranose
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150204 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Xylose isomerase Chain: A
Molecule details ›
Chain: A
Length: 388 amino acids
Theoretical weight: 43.28 KDa
Source organism: Streptomyces rubiginosus
UniProt:
  • Canonical: P24300 (Residues: 1-388; Coverage: 100%)
Gene name: xylA
Sequence domains: Xylose isomerase-like TIM barrel

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand NA 2 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID30B
Spacegroup: I222
Unit cell:
a: 93.99Å b: 99.38Å c: 103.1Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.122 0.121 0.13

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Citations

4 citation in other articles

Plug-and-play polymer microfluidic chips for hydrated, room temperature, fixed-target serial crystallography.
Gilbile et al. (2021)
3 more