› 6yd0

X-ray diffraction
1.95Å resolution

XFEL structure of the Soluble methane monooxygenase hydroxylase and regulatory subunit complex, from Methylosinus trichosporium OB3b, diferric state

Released:
DOI: 10.2210/pdb6yd0/pdb
Model geometry
Fit model/data
PDB entry 6yd0 coloured by chain, front view.
PDB entry 6yd0 coloured by chain, side view.
PDB entry 6yd0 coloured by chain, top view.
Source organism: Methylosinus trichosporium OB3b
Primary publication:
High-Resolution XFEL Structure of the Soluble Methane Monooxygenase Hydroxylase Complex with its Regulatory Component at Ambient Temperature in Two Oxidation States.
Srinivas V, Banerjee R, Lebrette H, Jones JC, Aurelius O, Kim IS, Pham CC, Gul S, Sutherlin KD, Bhowmick A, John J, Bozkurt E, Fransson T, Aller P, Butryn A, Bogacz I, Simon P, Keable S, Britz A, Tono K, Kim KS, Park SY, Lee SJ, Park J, Alonso-Mori R, Fuller FD, Batyuk A, Brewster AS, Bergmann U, Sauter NK, Orville AM, Yachandra VK, Yano J, Lipscomb JD, Kern J, Hogbom M
J. Am. Chem. Soc. 142 14249-14266 (2020)

Function and Biology Details

Reaction catalysed: 
Methane + NAD(P)H + O(2) = methanol + NAD(P)(+) + H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
PDBe Complex ID:
PDB-CPX-104203 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Methane monooxygenase Chain: B
Molecule details ›
Chain: B
Length: 395 amino acids
Theoretical weight: 45.3 KDa
Source organism: Methylosinus trichosporium OB3b
UniProt:
  • Canonical: A0A2D2D5X7 (Residues: 1-395; Coverage: 100%)
Gene name: CQW49_12475
Sequence domains: Methane/Phenol/Alkene Hydroxylase
Methane monooxygenase component A alpha chain Chain: D
Molecule details ›
Chain: D
Length: 526 amino acids
Theoretical weight: 60.03 KDa
Source organism: Methylosinus trichosporium OB3b
UniProt:
  • Canonical: P27353 (Residues: 1-526; Coverage: 100%)
Gene name: mmoX
Sequence domains: Methane/Phenol/Alkene Hydroxylase
Methane monooxygenase Chain: F
Methane monooxygenase regulatory protein B Chain: G

Ligands and Environments

2 bound ligands:
Ligand GOL 1 x GOL
Ligand FE 2 x FE
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SLAC LCLS BEAMLINE MFX
Spacegroup: P41212
Unit cell:
a: 106.891Å b: 106.891Å c: 303.962Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.163 0.162 0.19
Expression system: Escherichia coli

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