› 6ydc

X-ray diffraction
2Å resolution

X-ray structure of LPMO

Released:
DOI: 10.2210/pdb6ydc/pdb
PDB entry 6ydc coloured by chain, front view.
PDB entry 6ydc coloured by chain, side view.
PDB entry 6ydc coloured by chain, top view.
Source organism: Collariella virescens
Primary publication:
Oligosaccharide Binding and Thermostability of Two Related AA9 Lytic Polysaccharide Monooxygenases.
Tandrup T, Tryfona T, Frandsen KEH, Johansen KS, Dupree P, Lo Leggio L
Biochemistry 59 3347-3358 (2020)
PMID: 32818374

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-103960 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
AA9 family lytic polysaccharide monooxygenase A Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 252 amino acids
Theoretical weight: 27.79 KDa
Source organism: Collariella virescens
Expression system: Aspergillus oryzae
UniProt:
  • Canonical: A0A223GEC9 (Residues: 23-274; Coverage: 100%)
Gene name: AA9A
Sequence domains: Auxiliary Activity family 9 (formerly GH61)

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: BGC
Carbohydrate polymer
2 bound ligands:
Ligand CU 4 x CU
Ligand SO4 4 x SO4
1 modified residue:
Ligand HIC 4 x HIC

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE MASSIF-3
Spacegroup: P21
Unit cell:
a: 39.79Å b: 120.8Å c: 136.36Å
α: 90° β: 92.09° γ: 90°
R-values:
R R work R free
0.217 0.215 0.262
Expression system: Aspergillus oryzae

Quick links

Citations

3 review citations

Lytic polysaccharide monooxygenases and other histidine-brace copper proteins: structure, oxygen activation and biotechnological applications.
Ipsen et al. (2021)
2 more

1 mention without citation

Identification of the molecular determinants driving the substrate specificity of fungal lytic polysaccharide monooxygenases (LPMOs).
Frandsen et al. (2021)