› 6yde

X-ray diffraction
2.2Å resolution

X-ray structure of LPMO

Released:
DOI: 10.2210/pdb6yde/pdb
PDB entry 6yde coloured by chain, front view.
PDB entry 6yde coloured by chain, side view.
PDB entry 6yde coloured by chain, top view.
Source organism: Collariella virescens
Primary publication:
Oligosaccharide Binding and Thermostability of Two Related AA9 Lytic Polysaccharide Monooxygenases.
Tandrup T, Tryfona T, Frandsen KEH, Johansen KS, Dupree P, Lo Leggio L
Biochemistry 59 3347-3358 (2020)
PMID: 32818374

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-103960 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
AA9 family lytic polysaccharide monooxygenase A Chain: A
Molecule details ›
Chain: A
Length: 252 amino acids
Theoretical weight: 27.79 KDa
Source organism: Collariella virescens
Expression system: Aspergillus oryzae
UniProt:
  • Canonical: A0A223GEC9 (Residues: 23-274; Coverage: 100%)
Gene name: AA9A
Sequence domains: Auxiliary Activity family 9 (formerly GH61)

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC
Carbohydrate polymer
2 bound ligands:
Ligand SO4 1 x SO4
Ligand CU 1 x CU
1 modified residue:
Ligand HIC 1 x HIC

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE MASSIF-3
Spacegroup: P21212
Unit cell:
a: 39.55Å b: 124.37Å c: 51.67Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.194 0.264
Expression system: Aspergillus oryzae

Quick links

Citations

2 review citations

Metalloprotein catalysis: structural and mechanistic insights into oxidoreductases from neutron protein crystallography.
Schröder et al. (2021)
1 more

3 mentions without citation

Lytic polysaccharide monooxygenases and other histidine-brace copper proteins: structure, oxygen activation and biotechnological applications.
Ipsen et al. (2021)
2 more