6ydp

Electron Microscopy
3Å resolution

55S mammalian mitochondrial ribosome with mtEFG1 and P site fMet-tRNAMet (POST)

Released:
Source organisms:
Related structures: EMD-10778

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 94-mer (preferred)
PDBe Complex ID:
PDB-CPX-236629 (preferred)
Entry contents:
83 distinct polypeptide molecules
4 distinct RNA molecules
Macromolecules (87 distinct):
39S ribosomal protein L27, mitochondrial Chain: B0
Molecule details ›
Chain: B0
Length: 148 amino acids
Theoretical weight: 15.94 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A0A4X1U0F6 (Residues: 1-148; Coverage: 100%)
Sequence domains: Ribosomal L27 protein
39S ribosomal protein L20, mitochondrial isoform 1 Chain: BU
Molecule details ›
Chain: BU
Length: 149 amino acids
Theoretical weight: 17.55 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A0A286ZU56 (Residues: 1-149; Coverage: 100%)
Gene name: MRPL20
Sequence domains: Ribosomal protein L20
Large ribosomal subunit protein bL21m Chain: BV
Large ribosomal subunit protein uL22m Chain: BW
uL23m Chain: BX
Large ribosomal subunit protein uL24m Chain: BY
Large ribosomal subunit protein mL37 Chain: Ba
Large ribosomal subunit protein mL38 Chain: Bb
TGS domain-containing protein Chain: Bc
Large ribosomal subunit protein mL40 Chain: Bd
Mitochondrial ribosomal protein L41 Chain: Be
Large ribosomal subunit protein mL42 Chain: Bf
Large ribosomal subunit protein mL43 Chain: Bg
Large ribosomal subunit protein mL44 Chain: Bh
Large ribosomal subunit protein mL45 Chain: Bi
Large ribosomal subunit protein mL46 Chain: Bj
Small ribosomal subunit protein uS10 domain-containing protein Chain: Bk
Large ribosomal subunit protein mL49 Chain: Bl
Large ribosomal subunit protein mL50 Chain: Bm
Large ribosomal subunit protein mL51 Chain: Bn
Large ribosomal subunit protein mL52 Chain: Bo
mL53 Chain: Bp
Large ribosomal subunit protein mL54 Chain: Bq
Mitochondrial ribosomal protein L57 Chain: Bt
Large ribosomal subunit protein mL62 Chain: Bu
Large ribosomal subunit protein mL64 Chain: Bv
39S ribosomal protein S30, mitochondrial Chain: Bw
Large ribosomal subunit protein bL28m Chain: B1
Mitochondrial ribosomal protein S18A Chain: Bx
Mitochondrial ribosomal protein S2 Chain: AB
28S ribosomal protein S24, mitochondrial Chain: AC
Small ribosomal subunit protein uS5m Chain: AE
bS6m Chain: AF
Small ribosomal subunit protein uS7 domain-containing protein Chain: AG
Mitochondrial ribosomal protein S9 Chain: AI
Small ribosomal subunit protein uS10m Chain: AJ
Mitochondrial ribosomal protein S11 Chain: AK
Small ribosomal subunit protein uS12m Chain: AL
28S ribosomal protein S14, mitochondrial Chain: AN
Small ribosomal subunit protein uS15m Chain: AO
Small ribosomal subunit protein bS16m Chain: AP
uS17m Chain: AQ
Mitoribosomal protein bs18m, mrps18c Chain: AR
28S ribosomal protein S21, mitochondrial Chain: AU
unknown peptide Chain: AZ
Mitochondrial ribosomal protein S22 Chain: Aa
Small ribosomal subunit protein mS23 conserved domain-containing protein Chain: Ab
Small ribosomal subunit protein mS25 Chain: Ac
Progonadoliberin Chain: Ad
28S ribosomal protein S27, mitochondrial Chain: Ae
Mitochondrial ribosomal protein S28 Chain: Af
Large ribosomal subunit protein uL29m Chain: B2
Small ribosomal subunit protein mS29 Chain: Ag
Small ribosomal subunit protein mS31 Chain: Ah
Small ribosomal subunit protein mS33 Chain: Ai
Mitochondrial ribosomal protein S34 Chain: Aj
Small ribosomal subunit protein mS35 mitochondrial conserved domain-containing protein Chain: Ak
Coiled-coil-helix-coiled-coil-helix domain containing 1 Chain: Am
Small ribosomal subunit protein mS38 Chain: An
Small ribosomal subunit protein mS39 Chain: Ao
Small ribosomal subunit protein mS40 Chain: Ap
Mitochondrial ribosomal protein L12 Chains: CL, DL, EL, FL, GL, HL, LL
Large ribosomal subunit protein uL30m Chain: B3
Mitochondrial ribosomal protein L55 Chain: B4
Large ribosomal subunit protein bL32m Chain: B5
Large ribosomal subunit protein bL33m Chain: B6
Large ribosomal subunit protein bL34m Chain: B7
Large ribosomal subunit protein bL35m Chain: B8
Ribosomal protein Chain: B9
Elongation factor G, mitochondrial Chain: BC
Large ribosomal subunit protein uL2 RNA-binding domain-containing protein Chain: BD
Large ribosomal subunit protein uL3m Chain: BE
Large ribosomal subunit protein uL4m Chain: BF
Large ribosomal subunit protein bL9m Chain: BI
Large ribosomal subunit protein uL10m Chain: BJ
Large ribosomal subunit protein uL11m Chain: BK
Mitochondrial ribosomal protein L13 Chain: BN
Large ribosomal subunit protein uL14m Chain: BO
uL15m Chain: BP
Large ribosomal subunit protein uL16m Chain: BQ
Large ribosomal subunit protein bL17m Chain: BR
Mitochondrial ribosomal protein L18 Chain: BS
Large ribosomal subunit protein bL19m Chain: BT
12S rRNA Chains: AA, BA
fMet-tRNAMet (P site) Chain: AV
Messenger RNA Chain: AX
CP tRNAPhe Chain: BB

Ligands and Environments

No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution:
Relevant EMDB volumes: EMD-10778
Expression system: Escherichia coli