› 6yi0

X-ray diffraction
1.65Å resolution

Human histidine triad nucleotide-binding protein 2 (hHINT2) refined to 1.65 A in P41212 space group

Released:
DOI: 10.2210/pdb6yi0/pdb
PDB entry 6yi0 coloured by chain, front view.
PDB entry 6yi0 coloured by chain, side view.
PDB entry 6yi0 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Biochemical, crystallographic and biophysical characterization of histidine triad nucleotide-binding protein 2 with different ligands including a non-hydrolyzable analog of Ap4A.
Dolot R, Krakowiak A, Kaczmarek R, Włodarczyk A, Pichlak M, Nawrot B
Biochim Biophys Acta Gen Subj 1865 129968 (2021)
PMID: 34329705

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189782 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Adenosine 5'-monophosphoramidase HINT2 Chains: AAA, BBB, CCC, DDD
Molecule details ›
Chains: AAA, BBB, CCC, DDD
Length: 163 amino acids
Theoretical weight: 17.18 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9BX68 (Residues: 1-163; Coverage: 100%)
Gene name: HINT2
Sequence domains: HIT domain

Ligands and Environments

1 bound ligand:
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.2
Spacegroup: P41212
Unit cell:
a: 76.945Å b: 76.945Å c: 140.454Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.184 0.233
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 citations in other articles

Intracellular HINT1-Assisted Hydrolysis of Nucleoside 5'-O-Selenophosphate Leads to the Release of Hydrogen Selenide That Exhibits Toxic Effects in Human Cervical Cancer Cells.
Krakowiak et al. (2022)

1 mention without citation

Protective effect of HINT2 on mitochondrial function via repressing MCU complex activation attenuates cardiac microvascular ischemia-reperfusion injury.
Li et al. (2021)