› 6ylm

X-ray diffraction
1.6Å resolution

mCherry

Released:
DOI: 10.2210/pdb6ylm/pdb
PDB entry 6ylm coloured by chain, front view.
PDB entry 6ylm coloured by chain, side view.
PDB entry 6ylm coloured by chain, top view.
Source organism: Discosoma sp.
Primary publication:
Directionality of light absorption and emission in representative fluorescent proteins.
Myšková J, Rybakova O, Brynda J, Khoroshyy P, Bondar A, Lazar J
Proc Natl Acad Sci U S A 117 32395-32401 (2020)
PMID: 33273123

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112322 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fluorescent protein Chain: A
Molecule details ›
Chain: A
Length: 266 amino acids
Theoretical weight: 30.3 KDa
Source organism: Discosoma sp.
Expression system: Escherichia coli
UniProt:
  • Canonical: D1MPT3 (Residues: 1-236; Coverage: 100%)
Gene names: PAmCherry, PAmCherry1
Sequence domains: Green fluorescent protein

Ligands and Environments

1 bound ligand:
Ligand CL 8 x CL
1 modified residue:
Ligand NRQ 1 x NRQ

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: C2
Unit cell:
a: 107.28Å b: 42.92Å c: 85.77Å
α: 90° β: 128.5° γ: 90°
R-values:
R R work R free
0.201 0.201 0.216
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Visual pH Sensors: From a Chemical Perspective to New Bioengineered Materials.
Di Costanzo et al. (2021)
1 more

3 mentions without citation

Directionality of light absorption and emission in representative fluorescent proteins.
Myšková et al. (2020)
2 more