› 6ypx

X-ray diffraction
2.11Å resolution

Human histidine triad nucleotide-binding protein 2 (hHINT2) refined to 2.11 A in C2221 space group

Released:
DOI: 10.2210/pdb6ypx/pdb
PDB entry 6ypx coloured by chain, front view.
PDB entry 6ypx coloured by chain, side view.
PDB entry 6ypx coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Biochemical, crystallographic and biophysical characterization of histidine triad nucleotide-binding protein 2 with different ligands including a non-hydrolyzable analog of Ap4A.
Dolot R, Krakowiak A, Kaczmarek R, Włodarczyk A, Pichlak M, Nawrot B
Biochim Biophys Acta Gen Subj 1865 129968 (2021)
PMID: 34329705

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189782 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Adenosine 5'-monophosphoramidase HINT2 Chains: AAA, BBB, CCC, DDD
Molecule details ›
Chains: AAA, BBB, CCC, DDD
Length: 163 amino acids
Theoretical weight: 17.18 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9BX68 (Residues: 1-163; Coverage: 100%)
Gene name: HINT2
Sequence domains: HIT domain

Ligands and Environments

3 bound ligands:
Ligand CAC 2 x CAC
Ligand GOL 2 x GOL
Ligand NA 2 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Spacegroup: C2221
Unit cell:
a: 138.411Å b: 153.992Å c: 74.785Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.149 0.148 0.184
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 citations in other articles

Intracellular HINT1-Assisted Hydrolysis of Nucleoside 5'-O-Selenophosphate Leads to the Release of Hydrogen Selenide That Exhibits Toxic Effects in Human Cervical Cancer Cells.
Krakowiak et al. (2022)