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› 6yq3

X-ray diffraction
1.57Å resolution

Promiscuous Reductase LugOII Catalyzes Keto-reduction at C1 during Lugdunomycin Biosynthesis

Released:
DOI: 10.2210/pdb6yq3/pdb
Model geometry
Fit model/data
PDB entry 6yq3 coloured by chain, front view.
PDB entry 6yq3 coloured by chain, side view.
PDB entry 6yq3 coloured by chain, top view.
Source organism: Streptomyces sp. QL37
Primary publication:
Functional and Structural Insights into a Novel Promiscuous Ketoreductase of the Lugdunomycin Biosynthetic Pathway.
Xiao X, Elsayed SS, Wu C, van der Heul HU, Metsä-Ketelä M, Du C, Prota AE, Chen CC, Liu W, Guo RT, Abrahams JP, van Wezel GP
OpenAccess logo ACS Chem Biol 15 2529-2538 (2020)
PMID: 32840360

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-104492 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
FAD-binding domain-containing protein Chains: AAA, BBB
Molecule details ›
Chains: AAA, BBB
Length: 255 amino acids
Theoretical weight: 26.8 KDa
Source organism: Streptomyces sp. QL37
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: A0A2S6PN47 (Residues: 401-655; Coverage: 39%)
Gene name: C5F59_12925
Sequence domains: Enoyl-(Acyl carrier protein) reductase

Ligands and Environments

3 bound ligands:
Ligand NAP 2 x NAP
Ligand P7Q 2 x P7Q
Ligand EDO 2 x EDO
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: NSRRC BEAMLINE BL15A1
Unit cell:
a: 87.969Å b: 60.214Å c: 88.308Å
α: 90° β: 102.458° γ: 90°
R-values:
R R work R free
0.143 0.142 0.166
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Quick links

Citations

4 citation in other articles

Discovery of actinomycin L, a new member of the actinomycin family of antibiotics.
Machushynets et al. (2022)
3 more

1 mention without citation

Functional and Structural Insights into a Novel Promiscuous Ketoreductase of the Lugdunomycin Biosynthetic Pathway.
Xiao et al. (2020)