› 6yrw

X-ray diffraction
2.5Å resolution

SHMT from Streptococcus thermophilus Tyr55Ser variant as internal aldimine and as non-covalent complex with D-Ser

Released:
DOI: 10.2210/pdb6yrw/pdb
PDB entry 6yrw coloured by chain, front view.
PDB entry 6yrw coloured by chain, side view.
PDB entry 6yrw coloured by chain, top view.
Source organism: Streptococcus thermophilus
Entry authors: Petrillo G, Hernandez K, Bujons J, Clapes P, Uson I

Function and Biology Details

Reaction catalysed: 
5,10-methylenetetrahydrofolate + glycine + H(2)O = tetrahydrofolate + L-serine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177580 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine hydroxymethyltransferase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 416 amino acids
Theoretical weight: 45.06 KDa
Source organism: Streptococcus thermophilus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5M0B4 (Residues: 1-416; Coverage: 100%)
Gene names: glyA, str0755
Sequence domains: Serine hydroxymethyltransferase

Ligands and Environments


Cofactor: Ligand PLP 4 x PLP
2 bound ligands:
Ligand DSN 4 x DSN
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: C2
Unit cell:
a: 199.956Å b: 113.657Å c: 132.479Å
α: 90° β: 94.13° γ: 90°
R-values:
R R work R free
0.179 0.176 0.226
Expression system: Escherichia coli

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