› 6yuq

X-ray diffraction
1.95Å resolution

Capsule O-acetyltransferase of Neisseria meningitidis serogroup A in complex with polysaccharide

Released:
DOI: 10.2210/pdb6yuq/pdb
PDB entry 6yuq coloured by chain, front view.
PDB entry 6yuq coloured by chain, side view.
PDB entry 6yuq coloured by chain, top view.
Source organism: Neisseria meningitidis serogroup A
Primary publication:
Structural and mechanistic basis of capsule O-acetylation in Neisseria meningitidis serogroup A.
Fiebig T, Cramer JT, Bethe A, Baruch P, Curth U, Führing JI, Buettner FFR, Vogel U, Schubert M, Fedorov R, Mühlenhoff M
Nat Commun 11 4723 (2020)
PMID: 32948778

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130607 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
SacC Chains: A, B
Molecule details ›
Chains: A, B
Length: 255 amino acids
Theoretical weight: 29.37 KDa
Source organism: Neisseria meningitidis serogroup A
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O68216 (Residues: 1-247; Coverage: 100%)
Gene name: sacC
Sequence domains: Uncharacterised protein family (UPF0227)

Ligands and Environments

6 bound ligands:
Ligand EDO 1 x EDO
Ligand PEG 1 x PEG
Ligand CL 2 x CL
Ligand BMX 3 x BMX
Ligand BM3 1 x BM3
Ligand PGE 1 x PGE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Spacegroup: P41212
Unit cell:
a: 137.483Å b: 137.483Å c: 70.253Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.21 0.221
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Synthetic Glycans to Improve Current Glycoconjugate Vaccines and Fight Antimicrobial Resistance.
Del Bino et al. (2022)
2 more