› 6ywj

X-ray diffraction
2.4Å resolution

Arabidopsis aspartate transcarbamoylase mutant F161A complex with UMP

Released:
DOI: 10.2210/pdb6ywj/pdb
Model geometry
Fit model/data
PDB entry 6ywj coloured by chain, front view.
PDB entry 6ywj coloured by chain, side view.
PDB entry 6ywj coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Mechanisms of feedback inhibition and sequential firing of active sites in plant aspartate transcarbamoylase.
Bellin L, Del Caño-Ochoa F, Velázquez-Campoy A, Möhlmann T, Ramón-Maiques S
OpenAccess logo Nat Commun 12 947 (2021)
PMID: 33574254

Function and Biology Details

Reaction catalysed: 
Carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155860 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aspartate carbamoyltransferase, chloroplastic Chains: A, B
Molecule details ›
Chains: A, B
Length: 332 amino acids
Theoretical weight: 37.11 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P49077 (Residues: 82-390; Coverage: 79%)
Gene names: At3g20330, MQC12.7, PYRB
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand GOL 2 x GOL
Ligand U5P 2 x U5P
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
R-values:
R R work R free
0.141 0.138 0.19
Expression system: Escherichia coli BL21(DE3)

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Citations

1 review citation

Deciphering CAD: Structure and function of a mega-enzymatic pyrimidine factory in health and disease.
Del Caño-Ochoa et al. (2021)
9 other articles