› 6yy1

X-ray diffraction
3.06Å resolution

Arabidopsis aspartate transcarbamoylase in apo state

Released:
DOI: 10.2210/pdb6yy1/pdb
PDB entry 6yy1 coloured by chain, front view.
PDB entry 6yy1 coloured by chain, side view.
PDB entry 6yy1 coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Mechanisms of feedback inhibition and sequential firing of active sites in plant aspartate transcarbamoylase.
Bellin L, Del Caño-Ochoa F, Velázquez-Campoy A, Möhlmann T, Ramón-Maiques S
Nat Commun 12 947 (2021)
PMID: 33574254

Function and Biology Details

Reaction catalysed: 
Carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155860 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aspartate carbamoyltransferase, chloroplastic Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 332 amino acids
Theoretical weight: 37.19 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P49077 (Residues: 82-390; Coverage: 79%)
Gene names: At3g20330, MQC12.7, PYRB
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE MASSIF-1
Spacegroup: P212121
Unit cell:
a: 103.69Å b: 109.57Å c: 208.58Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.193 0.19 0.245
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

9 citation in other articles

A plastid nucleoside kinase is involved in inosine salvage and control of purine nucleotide biosynthesis.
Chen et al. (2023)
8 more

1 mention without citation

Deciphering CAD: Structure and function of a mega-enzymatic pyrimidine factory in health and disease.
Del Caño-Ochoa et al. (2021)