› 6z68

X-ray diffraction
1.35Å resolution

A novel metagenomic alpha/beta-fold esterase

Released:
DOI: 10.2210/pdb6z68/pdb
PDB entry 6z68 coloured by chain, front view.
PDB entry 6z68 coloured by chain, side view.
PDB entry 6z68 coloured by chain, top view.
Source organism: Pseudonocardia thermophila
Primary publication:
Crystal structures of a novel family IV esterase in free and substrate-bound form.
Höppner A, Bollinger A, Kobus S, Thies S, Coscolín C, Ferrer M, Jaeger KE, Smits SHJ
FEBS J 288 3570-3584 (2021)
PMID: 33342083

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-103621 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alpha/beta hydrolase fold-3 domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 376 amino acids
Theoretical weight: 40.51 KDa
Source organism: Pseudonocardia thermophila
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A1M6Y2K1 (Residues: 1-368; Coverage: 100%)
Gene name: SAMN05443637_118146
Sequence domains: alpha/beta hydrolase fold

Ligands and Environments

3 bound ligands:
Ligand PEG 2 x PEG
Ligand PG4 2 x PG4
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Unit cell:
a: 58.71Å b: 77.19Å c: 162.51Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.138 0.138 0.184
Expression system: Escherichia coli

Quick links

Citations

7 citation in other articles

A Plurizyme with Transaminase and Hydrolase Activity Catalyzes Cascade Reactions.
Roda et al. (2022)
6 more

1 mention without citation

Crystal structures of a novel family IV esterase in free and substrate-bound form.
Höppner et al. (2021)