6z6g

Electron Microscopy
3.06Å resolution

Cryo-EM structure of La Crosse virus polymerase at pre-initiation stage

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-107611 (preferred)
Entry contents:
1 distinct polypeptide molecule
3 distinct RNA molecules
Macromolecules (4 distinct):
RNA-directed RNA polymerase L Chain: A
Molecule details ›
Chain: A
Length: 2285 amino acids
Theoretical weight: 266.05 KDa
Source organism: La Crosse virus
Expression system: Trichoplusia ni
UniProt:
  • Canonical: A5HC98 (Residues: 1-2263; Coverage: 100%)
Gene name: L
Sequence domains:
5'vRNA 1-10 Chain: U
Molecule details ›
Chain: U
Length: 10 nucleotides
Theoretical weight: 3.22 KDa
Source organism: La Crosse orthobunyavirus
Expression system: Not provided
5'vRNA 9-16 Chain: X
Molecule details ›
Chain: X
Length: 8 nucleotides
Theoretical weight: 2.51 KDa
Source organism: La Crosse orthobunyavirus
Expression system: Not provided
3'vRNA 1-16 Chain: H
Molecule details ›
Chain: H
Length: 16 nucleotides
Theoretical weight: 5.06 KDa
Source organism: La Crosse orthobunyavirus
Expression system: Not provided

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.06Å
Relevant EMDB volumes: EMD-11093
Expression systems:
  • Trichoplusia ni
  • Not provided