› 6z6v

X-ray diffraction
2.19Å resolution

Globular head of C1q in complex with the nanobody C1qNb75

Released:
DOI: 10.2210/pdb6z6v/pdb
Model geometry
Fit model/data
PDB entry 6z6v coloured by chain, front view.
PDB entry 6z6v coloured by chain, side view.
PDB entry 6z6v coloured by chain, top view.
Source organisms:
Primary publication:
Functional and Structural Characterization of a Potent C1q Inhibitor Targeting the Classical Pathway of the Complement System.
Laursen NS, Pedersen DV, Gytz H, Zarantonello A, Bernth Jensen JM, Hansen AG, Thiel S, Andersen GR
OpenAccess logo Front Immunol 11 1504 (2020)
PMID: 32849513

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-208780 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Complement C1q subcomponent subunit A Chains: A, D
Molecule details ›
Chains: A, D
Length: 136 amino acids
Theoretical weight: 15.23 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P02745 (Residues: 110-245; Coverage: 61%)
Gene name: C1QA
Sequence domains: C1q domain
Complement C1q subcomponent subunit B Chains: B, E
Molecule details ›
Chains: B, E
Length: 141 amino acids
Theoretical weight: 16.16 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P02746 (Residues: 119-253; Coverage: 60%)
Gene name: C1QB
Sequence domains: C1q domain
Complement C1q subcomponent subunit C Chains: C, F
Nanobody C1q75 Chains: G, H

Ligands and Environments

1 bound ligand:
Ligand NAG 2 x NAG
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Unit cell:
a: 61.816Å b: 119.744Å c: 131.772Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.18 0.178 0.227
Expression systems:
  • Homo sapiens
  • Escherichia coli

Quick links

Citations

4 review citations

An in-depth analysis of the immunomodulatory mechanisms of intervertebral disc degeneration.
Song et al. (2022)
3 more

3 mentions without citation

Nanobodies Provide Insight into the Molecular Mechanisms of the Complement Cascade and Offer New Therapeutic Strategies.
Zarantonello et al. (2021)
2 more