› 6z70

X-ray diffraction
2Å resolution

Structure of the MATE family multidrug resistance transporter Aq_128 from Aquifex aeolicus in the outward-facing state

Released:
DOI: 10.2210/pdb6z70/pdb
PDB entry 6z70 coloured by chain, front view.
PDB entry 6z70 coloured by chain, side view.
PDB entry 6z70 coloured by chain, top view.
Source organism: Aquifex aeolicus VF5
Primary publication:
The structure of the Aquifex aeolicus MATE family multidrug resistance transporter and sequence comparisons suggest the existence of a new subfamily.
Zhao J, Xie H, Mehdipour AR, Safarian S, Ermler U, Münke C, Thielmann Y, Hummer G, Ebersberger I, Wang J, Michel H
Proc Natl Acad Sci U S A 118 (2021)
PMID: 34753818

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130328 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Multidrug-efflux transporter Chains: A, B
Molecule details ›
Chains: A, B
Length: 496 amino acids
Theoretical weight: 55.57 KDa
Source organism: Aquifex aeolicus VF5
Expression system: Escherichia coli
UniProt:
  • Canonical: O66528 (Residues: 1-472; Coverage: 100%)
Gene name: aq_128
Sequence domains: MatE

Ligands and Environments

1 bound ligand:
Ligand OLC 7 x OLC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P212121
Unit cell:
a: 72.1Å b: 116.1Å c: 137.6Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.266 0.266 0.284
Expression system: Escherichia coli

Quick links

Citations

2 review citations

CHARMM-GUI Membrane Builder: Past, Current, and Future Developments and Applications.
Feng et al. (2023)
1 more

1 mention without citation

The structure of the Aquifex aeolicus MATE family multidrug resistance transporter and sequence comparisons suggest the existence of a new subfamily.
Zhao et al. (2021)