› 6z81

X-ray diffraction
2.31Å resolution

Function and Biology Details

Reaction catalysed: 
L-threonylcarbamoyladenylate + adenine(37) in tRNA = AMP + N(6)-L-threonylcarbamoyladenine(37) in tRNA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138800 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
tRNA N6-adenosine threonylcarbamoyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 343 amino acids
Theoretical weight: 36.88 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: P05852 (Residues: 1-337; Coverage: 100%)
Gene names: JW3036, b3064, gcp, tsaD, ygjD
Sequence domains: tRNA N6-adenosine threonylcarbamoyltransferase
tRNA threonylcarbamoyladenosine biosynthesis protein TsaB Chains: C, D
Molecule details ›
Chains: C, D
Length: 237 amino acids
Theoretical weight: 26.03 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: P76256 (Residues: 1-231; Coverage: 100%)
Gene names: JW1796, b1807, tsaB, yeaZ
Sequence domains: tRNA N6-adenosine threonylcarbamoyltransferase

Ligands and Environments

8 bound ligands:
Ligand NI 4 x NI
Ligand ZN 2 x ZN
Ligand QCB 2 x QCB
Ligand SO4 4 x SO4
Ligand NA 3 x NA
Ligand ACT 9 x ACT
Ligand PEG 3 x PEG
Ligand AMP 2 x AMP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P1
Unit cell:
a: 63.23Å b: 69.25Å c: 86.54Å
α: 109.38° β: 92° γ: 116.94°
R-values:
R R work R free
0.185 0.182 0.233
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Quick links

Citations

2 review citations

Conservation and Diversification of tRNA t6A-Modifying Enzymes across the Three Domains of Life.
Su et al. (2022)
1 more

4 mentions without citation

The structural and functional workings of KEOPS.
Beenstock et al. (2021)
3 more