› 6zea

X-ray diffraction
1.54Å resolution

Strictosidine Synthase from Catharanthus roseus in complex with racemic 1-methyl-2,3,4,9-tetrahydro-1H-beta-carboline

Released:
DOI: 10.2210/pdb6zea/pdb
PDB entry 6zea coloured by chain, front view.
PDB entry 6zea coloured by chain, side view.
PDB entry 6zea coloured by chain, top view.
Source organism: Catharanthus roseus
Entry authors: Eger E, Sharma M, Kroutil W, Grogan G

Function and Biology Details

Reaction catalysed: 
3-alpha-(S)-strictosidine + H(2)O = tryptamine + secologanin
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148362 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Strictosidine synthase Chain: A
Molecule details ›
Chain: A
Length: 321 amino acids
Theoretical weight: 35.76 KDa
Source organism: Catharanthus roseus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P18417 (Residues: 32-352; Coverage: 100%)
Gene names: SSS, STR1
Sequence domains: Strictosidine synthase

Ligands and Environments

3 bound ligands:
Ligand EDO 4 x EDO
Ligand QGH 1 x QGH
Ligand QGK 1 x QGK
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P212121
Unit cell:
a: 44.44Å b: 71.42Å c: 109.58Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 0.182 0.218
Expression system: Escherichia coli BL21(DE3)

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