› 6zh1

X-ray diffraction
2.2Å resolution

Crystal structure of complex between FH19-20 and FhbA protein from Borrelia hermsii

Released:
DOI: 10.2210/pdb6zh1/pdb
PDB entry 6zh1 coloured by chain, front view.
PDB entry 6zh1 coloured by chain, side view.
PDB entry 6zh1 coloured by chain, top view.
Source organisms:
Primary publication:
Mechanism of Borrelia immune evasion by FhbA-related proteins.
Kogan K, Haapasalo K, Kotila T, Moore R, Lappalainen P, Goldman A, Meri T
PLoS Pathog 18 e1010338 (2022)
PMID: 35303742

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140219 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
FhbA protein Chain: A
Molecule details ›
Chain: A
Length: 186 amino acids
Theoretical weight: 22.15 KDa
Source organism: Borrelia nietonii YOR
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: W5SB08 (Residues: 17-17, 31-202; Coverage: 86%)
Gene name: BHY_1174
Complement factor H Chain: B
Molecule details ›
Chain: B
Length: 133 amino acids
Theoretical weight: 15.08 KDa
Source organism: Homo sapiens
Expression system: Komagataella pastoris
UniProt:
  • Canonical: P08603 (Residues: 1104-1231; Coverage: 11%)
Gene names: CFH, HF, HF1, HF2
Sequence domains: Sushi repeat (SCR repeat)

Ligands and Environments

2 bound ligands:
Ligand EDO 4 x EDO
Ligand SO4 5 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P212121
Unit cell:
a: 43.273Å b: 60.327Å c: 145.681Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.193 0.19 0.24
Expression systems:
  • Escherichia coli BL21(DE3)
  • Komagataella pastoris

Quick links

Citations

1 mention without citation

Mechanism of Borrelia immune evasion by FhbA-related proteins.
Kogan et al. (2022)