› 6zhc

X-ray diffraction
1.92Å resolution

PROTAC6 mediated complex of VHL:EloB:EloC and Bcl-xL

Released:
DOI: 10.2210/pdb6zhc/pdb
PDB entry 6zhc coloured by chain, front view.
PDB entry 6zhc coloured by chain, side view.
PDB entry 6zhc coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural Insights into PROTAC-Mediated Degradation of Bcl-xL.
Chung CW, Dai H, Fernandez E, Tinworth CP, Churcher I, Cryan J, Denyer J, Harling JD, Konopacka A, Queisser MA, Tame CJ, Watt G, Jiang F, Qian D, Benowitz AB
ACS Chem Biol 15 2316-2323 (2020)
PMID: 32697072

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-154083 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
von Hippel-Lindau disease tumor suppressor Chain: AAA
Molecule details ›
Chain: AAA
Length: 155 amino acids
Theoretical weight: 18.01 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P40337 (Residues: 59-213; Coverage: 73%)
Gene name: VHL
Sequence domains:
Elongin-B Chain: BBB
Molecule details ›
Chain: BBB
Length: 107 amino acids
Theoretical weight: 12.09 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q15370 (Residues: 1-107; Coverage: 91%)
Gene names: ELOB, TCEB2
Sequence domains: Ubiquitin family
Elongin-C Chain: CCC
Molecule details ›
Chain: CCC
Length: 96 amino acids
Theoretical weight: 10.84 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q15369 (Residues: 17-112; Coverage: 86%)
Gene names: ELOC, TCEB1
Sequence domains: Skp1 family, tetramerisation domain
Bcl-2-like protein 1 Chain: DDD
Molecule details ›
Chain: DDD
Length: 221 amino acids
Theoretical weight: 24.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q07817 (Residues: 1-209; Coverage: 90%)
Gene names: BCL2L, BCL2L1, BCLX
Sequence domains:

Ligands and Environments

4 bound ligands:
Ligand QL8 1 x QL8
Ligand EDO 17 x EDO
Ligand GOL 9 x GOL
Ligand IOD 12 x IOD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P212121
Unit cell:
a: 70.99Å b: 101.18Å c: 106.93Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.171 0.169 0.196
Expression system: Escherichia coli

Quick links

Citations

10 review citations

Major advances in targeted protein degradation: PROTACs, LYTACs, and MADTACs.
Alabi et al. (2021)
9 more

4 mentions without citation

Advancing targeted protein degradation for cancer therapy.
Dale et al. (2021)
3 more