› 6zhj

Electron crystallography
3.26Å resolution

3D electron diffraction structure of thermolysin from Bacillus thermoproteolyticus

Released:
DOI: 10.2210/pdb6zhj/pdb
PDB entry 6zhj coloured by chain, front view.
PDB entry 6zhj coloured by chain, side view.
PDB entry 6zhj coloured by chain, top view.
Source organism: Bacillus thermoproteolyticus
Primary publication:
Statistically correcting dynamical electron scattering improves the refinement of protein nanocrystals, including charge refinement of coordinated metals.
Blum TB, Housset D, Clabbers MTB, van Genderen E, Bacia-Verloop M, Zander U, McCarthy AA, Schoehn G, Ling WL, Abrahams JP
Acta Crystallogr D Struct Biol 77 75-85 (2021)
PMID: 33404527

Function and Biology Details

Reaction catalysed: 
Preferential cleavage: -|-Leu > -|-Phe.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133580 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thermolysin Chain: A
Molecule details ›
Chain: A
Length: 316 amino acids
Theoretical weight: 34.36 KDa
Source organism: Bacillus thermoproteolyticus
Expression system: Not provided
UniProt:
  • Canonical: P00800 (Residues: 233-548; Coverage: 61%)
Gene name: npr
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand ZN 1 x ZN
Ligand CA 4 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.26Å
Expression system: Not provided

Quick links

Citations

2 review citations

Better, Faster, Cheaper: Recent Advances in Cryo-Electron Microscopy.
Chua et al. (2022)
1 more

2 mentions without citation

Electron Diffraction of 3D Molecular Crystals.
Saha et al. (2022)
1 more